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lab 97 image gauge software (version 2.54  (FUJIFILM)
90
FUJIFILM lab 97 image gauge software (version 2.54
Akt and GSK-3β regulation by cadmium. SH-SY5Y cells were treated with 25 μM Cd for the indicated times. Total cellular proteins were extracted and separated using SDS-PAGE. a – Western blot analysis was performed using anti-phospho-Akt (T308), S473 and phospho-GSK-3β (S9) antibodies. Band intensities were quantified based on densitometric values using Fujifilm Science <t>Lab</t> <t>97</t> Image Gauge software (version 2.54). The data are from at least three independent experiments. b , c – p-AKT and p-GSK-3β were analyzed via western blotting after 30 min pretreatment with 10 μM BAPTA-AM ( b ) or 1 μM wortmannin ( c ), followed by 12 h incubation with 25 μM Cd. d – Apoptotic cells were counted using the annexin V assay. The data are expressed as the means ± SEM of the percentage of apoptotic cells from at least three independent experiments. * p < 0.05, ** p < 0.01
Lab 97 Image Gauge Software (Version 2.54, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Akt and GSK-3β regulation by cadmium. SH-SY5Y cells were treated with 25 μM Cd for the indicated times. Total cellular proteins were extracted and separated using SDS-PAGE. a – Western blot analysis was performed using anti-phospho-Akt (T308), S473 and phospho-GSK-3β (S9) antibodies. Band intensities were quantified based on densitometric values using Fujifilm Science Lab 97 Image Gauge software (version 2.54). The data are from at least three independent experiments. b , c – p-AKT and p-GSK-3β were analyzed via western blotting after 30 min pretreatment with 10 μM BAPTA-AM ( b ) or 1 μM wortmannin ( c ), followed by 12 h incubation with 25 μM Cd. d – Apoptotic cells were counted using the annexin V assay. The data are expressed as the means ± SEM of the percentage of apoptotic cells from at least three independent experiments. * p < 0.05, ** p < 0.01

Journal: Cellular & Molecular Biology Letters

Article Title: GSK-3β-mediated regulation of cadmium-induced cell death and survival

doi: 10.1186/s11658-018-0076-2

Figure Lengend Snippet: Akt and GSK-3β regulation by cadmium. SH-SY5Y cells were treated with 25 μM Cd for the indicated times. Total cellular proteins were extracted and separated using SDS-PAGE. a – Western blot analysis was performed using anti-phospho-Akt (T308), S473 and phospho-GSK-3β (S9) antibodies. Band intensities were quantified based on densitometric values using Fujifilm Science Lab 97 Image Gauge software (version 2.54). The data are from at least three independent experiments. b , c – p-AKT and p-GSK-3β were analyzed via western blotting after 30 min pretreatment with 10 μM BAPTA-AM ( b ) or 1 μM wortmannin ( c ), followed by 12 h incubation with 25 μM Cd. d – Apoptotic cells were counted using the annexin V assay. The data are expressed as the means ± SEM of the percentage of apoptotic cells from at least three independent experiments. * p < 0.05, ** p < 0.01

Article Snippet: Band intensities were quantified based on densitometric values using Fujifilm Science Lab 97 Image Gauge software (version 2.54).

Techniques: SDS Page, Western Blot, Software, Incubation, Annexin V Assay